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Image Search Results


Journal: Cell Reports

Article Title: Human GBP1 Differentially Targets Salmonella and Toxoplasma to License Recognition of Microbial Ligands and Caspase-Mediated Death

doi: 10.1016/j.celrep.2020.108008

Figure Lengend Snippet:

Article Snippet: To create the caspase-8-3xFlag expressing Dox-inducible plasmid (pLenti-Tet- CASP8 -3xFlag), the empty vector backbone was digested with BamHI, CASP8 ORF was amplified from pcDNA3- CASP8 by PCR (Addgene #11817, a gift from Guy Salvesen) , 3xFlag was amplified from LentiCRISPRv2 (Addgene #52961, a gift from Feng Zhang) ( ) and all fragments assembled with a Gibson assembly.

Techniques: Virus, Recombinant, Apoptosis Assay, Enzyme-linked Immunosorbent Assay, Bicinchoninic Acid Protein Assay, Silver Staining, cDNA Synthesis, SYBR Green Assay, Imaging, Labeling, Molecular Cloning, Negative Control, Plasmid Preparation, Software